PREPARATION OF [4R-H-3]NADH, [4R-H-3]NADPH AND THE CORRESPONDING 4S-ISOMERS ALL WITH SUBSTANTIAL SPECIFIC ACTIVITIES

被引：7
|
作者：
ICHINOSE, K [1 ]
LEEPER, FJ [1 ]
BATTERSBY, AR [1 ]
机构：
[1] UNIV CAMBRIDGE,CHEM LAB,LENSFIELD RD,CAMBRIDGE CB2 1EW,ENGLAND
来源：
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1993年 / 11期
关键词：
D O I：
10.1039/p19930001213
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Methods are developed for the enzymic preparation of [4R-H-3]NADH, [4R-H-3]NADPH and the corresponding 4S-isomers, all stereospecifically labelled and of specific activities amply high enough for sensitive labelling experiments. Sodium borotritiide provides the tritium for all four cofactors.
引用
收藏
页码：1213 / 1216
页数：4
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