PREPARATION OF [4R-H-3]NADH, [4R-H-3]NADPH AND THE CORRESPONDING 4S-ISOMERS ALL WITH SUBSTANTIAL SPECIFIC ACTIVITIES

被引:7
|
作者
ICHINOSE, K [1 ]
LEEPER, FJ [1 ]
BATTERSBY, AR [1 ]
机构
[1] UNIV CAMBRIDGE,CHEM LAB,LENSFIELD RD,CAMBRIDGE CB2 1EW,ENGLAND
关键词
D O I
10.1039/p19930001213
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Methods are developed for the enzymic preparation of [4R-H-3]NADH, [4R-H-3]NADPH and the corresponding 4S-isomers, all stereospecifically labelled and of specific activities amply high enough for sensitive labelling experiments. Sodium borotritiide provides the tritium for all four cofactors.
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页码:1213 / 1216
页数:4
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