A more efficient extraction method of human bone resulting in improved DNA profiling

被引：4
|
作者：
Desmyter, Stijn [1 ]
De Greef, Christine [1 ]
机构：
[1] Natl Inst Criminalist & Criminol, Vilvoordsesteenweg 100, B-1120 Brussels, Belgium
来源：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2008年 / 1卷 / 01期
关键词：
Bone; Teeth; DNA extraction; DNA profiling;
D O I：
10.1016/j.fsigss.2007.10.092
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Eight human bone samples, from a forensic case, were extracted in parallel using our standard protocol with and without PTB in the buffer. Both methods were sometimes inadequate for (complete) STR profiling, while the presence of PTB even decreases the DNA yield. The complete decalcification of the bone extraction residues in an EDTA-solution with SDS recovered sufficient amounts of DNA, which resulted in complete STR profiling for all samples. Complete decalcification without SDS yielded even higher amounts of DNA and also complete STR profiling for all samples. Similar results were obtained for the DNA extraction from a human tooth. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：24 / 25
页数：2
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