IMPROVED METHOD FOR DNA EXTRACTION FROM VITIS-VINIFERA

被引：0
|
作者：
STEENKAMP, J [1 ]
WIID, I [1 ]
LOURENS, A [1 ]
VANHELDEN, P [1 ]
机构：
[1] UNIV STELLENBOSCH,DEPT MED BIOCHEM,CTR MOLEC & CELLULAR BIOL,MED RES COUNCIL,TYGERBERG 7505,SOUTH AFRICA
来源：
AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE | 1994年 / 45卷 / 01期
关键词：
VITIS; GRAPEVINES; DNA; FINGERPRINTING;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An inexpensive and high yielding procedure is described for the isolation of DNA from small quantities of leaves or roots from Vitis vinifera. The buffer capacity of the extraction solution was found to be the single most important factor in the isolation of the DNA. Different detergent (CTAB) concentrations have an effect on the efficiency of cleavage by restriction endonucleases. The quality of the DNA was checked by fingerprint analysis after digestion with HaeIII and HinfI restriction enzymes and probing with the multilocus pV47-2 probe. Differences between Vitis vinifera cultivars were detected.
引用
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页码：102 / 106
页数：5
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