An enzyme-linked immunosorbent assay [ELISA] for the detection of antibodies [Ab] to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The ELISA can be specific for human immunoglobulin (Ig) M Ab when a .mu.-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue IgM Ab were demonstrated in a [human] infant suspected of having a recent dengue infection.
机构:
Univ Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, MalaysiaUniv Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, Malaysia
Lam, SK
Ew, CL
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机构:Univ Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, Malaysia
Ew, CL
Mitchell, JL
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机构:Univ Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, Malaysia
Mitchell, JL
Cuzzubbo, AJ
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机构:Univ Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, Malaysia
Cuzzubbo, AJ
Devine, PL
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机构:Univ Malaya, Fac Med, Dept Med Microbiol, WHO Collaborating Ctr Arbovirus Ref & Res Dengue, Kuala Lumpur 50603, Malaysia
机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Nakayama, Eri
Yokoyama, Ayaka
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机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Yokoyama, Ayaka
Miyamoto, Hiroko
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机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Miyamoto, Hiroko
Igarashi, Manabu
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机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Igarashi, Manabu
Kishida, Noriko
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机构:
Natl Inst Infect Dis, Ctr Influenza Virus Res, Lab Influenza Virus Surveillance, Tokyo, JapanHokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Kishida, Noriko
Matsuno, Keita
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机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Matsuno, Keita
Marzi, Andrea
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机构:
NIAID, Virol Lab, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USAHokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Marzi, Andrea
Feldmann, Heinz
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机构:
NIAID, Virol Lab, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USAHokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Feldmann, Heinz
Ito, Kimihito
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机构:Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Ito, Kimihito
Saijo, Masayuki
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机构:
Natl Inst Infect Dis, Dept Virol 1, Tokyo, JapanHokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan
Saijo, Masayuki
Takada, Ayato
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机构:
Hokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, JapanHokkaido Univ, Res Ctr Zoonosis Control, Dept Global Epidemiol, Kita Ku, Sapporo, Hokkaido 0010020, Japan