ISOLATION AND CHARACTERIZATION OF GROUP-I ISOALLERGENS FROM BERMUDA GRASS-POLLEN

被引:30
|
作者
SMITH, PM
AVJIOGLU, A
WARD, LR
SIMPSON, RJ
KNOX, RB
SINGH, MB
机构
[1] UNIV MELBOURNE,SCH BOT,PARKVILLE,VIC 3052,AUSTRALIA
[2] JOHNS HOPKINS UNIV,CTR ALLERGY,BALTIMORE,MD
[3] LUDWIG INST CANC RES,JOINT PROT STRUCT LAB,PARKVILLE,VIC,AUSTRALIA
[4] WALTER & ELISA HALL INST MED RES,PARKVILLE,VIC,AUSTRALIA
关键词
POLLEN ALLERGEN; BERMUDA GRASS; CYN D I;
D O I
10.1159/000236709
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Two isoallergens of Cyn d I were isolated using preparative isoelectric focussing, and were designated Cyn d Ia and b. These isoallergens differ in their pI, molecular weight (Cyn d Ia, 32 kD and Cyn d Ib, 31 kD) and their NH2-terminal sequence. Four monoclonal antibodies (Mabs) were produced using Cyn d Ia as antigen. These Mabs recognized both Cyn d Ia and b. One of the Pollen Allergen Mabs recognized four different pI forms of Cyn d I on 2D gels. The Mabs also Bermuda grass recognized cross-reactive epitopes on proteins from eight other grasses including rye grass, timothy grass, Kentucky bluegrass and Johnson grass.
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页码:57 / 64
页数:8
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