Two isoallergens of Cyn d I were isolated using preparative isoelectric focussing, and were designated Cyn d Ia and b. These isoallergens differ in their pI, molecular weight (Cyn d Ia, 32 kD and Cyn d Ib, 31 kD) and their NH2-terminal sequence. Four monoclonal antibodies (Mabs) were produced using Cyn d Ia as antigen. These Mabs recognized both Cyn d Ia and b. One of the Pollen Allergen Mabs recognized four different pI forms of Cyn d I on 2D gels. The Mabs also Bermuda grass recognized cross-reactive epitopes on proteins from eight other grasses including rye grass, timothy grass, Kentucky bluegrass and Johnson grass.
机构:Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan
Wu, WC
Tam, MF
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机构:Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan
Tam, MF
Peng, HJ
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机构:Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan
Peng, HJ
Tsai, LC
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机构:Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan
Tsai, LC
Chi, CW
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机构:Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan
Chi, CW
Chang, ZN
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Natl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, TaiwanNatl Yang Ming Univ, Fac Med Technol, Inst Biotechnol Med, Taipei 112, Taiwan