HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF OLIGOSACCHARIDE ALDITOLS AND GLYCOPEPTIDES ON A GRAPHITIZED CARBON COLUMN

被引：72
|
作者：
DAVIES, M [1 ]
SMITH, KD [1 ]
HARBIN, AM [1 ]
HOUNSELL, EF [1 ]
机构：
[1] CLIN RES CTR, GLYCOCONJUGATES SECT, WATFORD RD, HARROW HA1 3UJ, MIDDX, ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 609卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)80155-N
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The chromatographic behaviour of oligosaccharide alditols and glycopeptides containing neutral and acetamido sugars and sialic acid has been investigated on a HyperCarb porous graphitised carbon column. The alditols were substantially retained and could be eluted in 0-25% acetonitrile-0.05% trifluoroacetic acid in 0.05% aqueous trifluoroacetic acid between 3-30 min for mono- to hexasaccharides. Elution patterns were based on both size, charge and linkage such that isomeric compounds could be separated from each other.
引用
收藏
页码：125 / 131
页数：7
相关论文
共 50 条
  • [21] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOSPHOLIPIDS ON AMINOPROPYL SILICA COLUMN
    SHIMBO, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (10): : 2643 - 2645
  • [22] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOSPHOLIPIDS ON AMINOPROPYL SILICA COLUMN
    VORONIN, MV
    VASILENKO, IA
    SHVETZ, VI
    BIOORGANICHESKAYA KHIMIYA, 1989, 15 (03): : 423 - 424
  • [23] PRE-COLUMN DERIVATIZATION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    ROSS, MSF
    JOURNAL OF CHROMATOGRAPHY, 1977, 141 (01): : 107 - 119
  • [24] ANALYSIS OF TIOCONAZOLE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A POROUS GRAPHITIC CARBON COLUMN
    BERRIDGE, JC
    JOURNAL OF CHROMATOGRAPHY, 1988, 449 (01): : 317 - 321
  • [25] THE ROLE OF THE COLUMN IN ROUTINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC)
    KARGER, BL
    JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1981, 19 (08): : 561 - 562
  • [26] PURIFICATION OF GLYCOPEPTIDES OF HUMAN-PLASMA PROTEINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    TAKAHASHI, N
    TAKAHASHI, Y
    ORTEL, TL
    LOZIER, JN
    ISHIOKA, N
    PUTNAM, FW
    JOURNAL OF CHROMATOGRAPHY, 1984, 317 (DEC): : 11 - 26
  • [27] SEPARATION OF MUCIN OLIGOSACCHARIDE-ALDITOLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON ALKYLAMINE-BONDED SILICA COLUMNS - EFFECTS OF STRUCTURAL PARAMETERS
    KLEIN, A
    CARNOY, C
    LOGUIDICE, JM
    LAMBLIN, G
    ROUSSEL, P
    CARBOHYDRATE RESEARCH, 1992, 236 (236) : 9 - 16
  • [28] NEW CARBON ADSORBENT FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    PLZAK, Z
    DOUSEK, FP
    JANSTA, J
    JOURNAL OF CHROMATOGRAPHY, 1978, 147 (JAN): : 137 - 142
  • [29] HIGH-PERFORMANCE LIQUID LIQUID-CHROMATOGRAPHY
    CROMBEEN, JP
    HEEMSTRA, S
    KRAAK, JC
    JOURNAL OF CHROMATOGRAPHY, 1984, 286 (MAR): : 119 - 129
  • [30] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    KARLESKIND, A
    REVUE FRANCAISE DES CORPS GRAS, 1977, 24 (8-9): : 419 - 425
12345