A SIMPLE METHOD FOR THE DIRECT EXTRACTION OF PLASMID DNA FROM YEAST

被引：6
|
作者：
SOBANSKI, MA
DICKINSON, JR
机构：
[1] School of Pure and Applied Biology, University of Wales College of Cardiff, Cardiff, CF1 3TL
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 03期
关键词：
D O I：
10.1007/BF00157083
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and simple method for the small scale isolation of shuttle plasmid DNA from Saccharomyces cerevisiae is described. It uses glass beads to break cells and reagents which are also used in bacterial mini-preps to yield plasmid DNA without chromosomal contamination in sufficient quantities to enable direct visualisation on agarose gels.
引用
收藏
页码：225 / 230
页数：6
相关论文
共 50 条
  • [41] Simple method for DNA extraction from pancreatic juice for PCR amplification assays
    Petra Müller
    Ralf Jesnowski
    Stefan Liebe
    Arndt Rolfs
    Matthias Löhr
    International Journal of Pancreatology, 1999, 25 : 39 - 43
  • [42] A simple and efficient method for PCR amplifiable DNA extraction from ancient bones
    Kalmar, Tibor
    Bachrati, Csanad Z.
    Marcsik, Antonia
    Rasko, Istvan
    NUCLEIC ACIDS RESEARCH, 2000, 28 (12) : 67
  • [43] SCREENMAX(TM) PLASMID MINI-PREP - SUPER RAPID PLASMID DNA EXTRACTION METHOD
    KADOKAMI, Y
    COLGIN, MA
    YESGAR, PW
    LEWIS, RV
    BIOTECHNIQUES, 1994, 17 (03) : 580 - 584
  • [44] DIRECT PLASMID SHUTTLING FROM YEAST INTO ESCHERICHIA-COLI BY ELECTRODUCTION
    NACKEN, V
    DEGRYSE, E
    ACHSTETTER, T
    NUCLEIC ACIDS RESEARCH, 1994, 22 (08) : 1509 - 1510
  • [45] A SIMPLE METHOD FOR THE PREPARATION OF PLASMID AND CHROMOSOMAL ESCHERICHIA-COLI DNA
    GRIMBERG, J
    MAGUIRE, S
    BELLUSCIO, L
    NUCLEIC ACIDS RESEARCH, 1989, 17 (21) : 8893 - 8893
  • [46] A SIMPLE METHOD FOR THE PREPARATION OF THE COVALENTLY CLOSED CIRCULAR FORM OF PLASMID DNA
    LEBRUN, JJ
    RENTIERDELRUE, F
    MERCIER, L
    BIOTECHNIQUES, 1988, 6 (09) : 834 - &
  • [47] A simple and inexpensive method for sending binary vector plasmid DNA by mail
    Thomson, James G.
    Thilmony, Roger
    PLANT BIOTECHNOLOGY, 2008, 25 (04) : 403 - 406
  • [48] A simple method for screening bacterial colonies for mutagenized sites in plasmid DNA
    Rodríguez-Lecompte, JC
    Nagarajan, MN
    Kibenge, FSB
    JOURNAL OF VIROLOGICAL METHODS, 2002, 99 (1-2) : 93 - 98
  • [49] A NOVEL METHOD FOR TRANSFORMATION OF INTACT YEAST-CELLS BY ELECTROINJECTION OF PLASMID DNA
    HASHIMOTO, H
    MORIKAWA, H
    YAMADA, Y
    KIMURA, A
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 21 (05) : 336 - 339
  • [50] A simple and efficient method for extraction of Taq DNA polymerase
    Chen, Sique
    Zheng, Xiujuan
    Cao, Hongrui
    Jiang, Linghui
    Liu, Fangqian
    Sun, Xinli
    ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2015, 18 (05): : 355 - 358
12345