Simple method for DNA extraction from pancreatic juice for PCR amplification assays

被引：0
|
作者：
Petra Müller
Ralf Jesnowski
Stefan Liebe
Arndt Rolfs
Matthias Löhr
机构：
[1] University of Rostock,Division of Gastroenterology, Department of Medicine
[2] University of Rostock,Department of Neurology
来源：
International Journal of Pancreatology | 1999年 / 25卷
关键词：
PCR; pancreatic juice; DNA; ras; DNA preparation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Conclusion: Preparation of DNA from pancreatic juice for subsequent polymerase chain reaction (PCR) is difficult, but manageable. The protocol presented offers a simple and fast solution. This method might be applicable to other complicated samples, such as saliva, wound secretions, or stool washings.
引用
收藏
页码：39 / 43
页数：4
相关论文
共 50 条
  • [1] Simple method for DNA extraction from pancreatic juice for PCR amplification assays
    Müller, P
    Jesnowski, R
    Liebe, S
    Rolfs, A
    Löhr, M
    INTERNATIONAL JOURNAL OF PANCREATOLOGY, 1999, 25 (01) : 39 - 43
  • [2] SCHISTOSOMA-MANSONI - A SIMPLE METHOD FOR THE EXTRACTION OF DNA FROM SINGLE WORMS FOR PCR AMPLIFICATION
    CHARRIERFERRARA, S
    DJABALI, M
    GOUDOTCROZEL, V
    EXPERIMENTAL PARASITOLOGY, 1991, 73 (03) : 384 - 384
  • [3] A PCR amplification method without DNA extraction
    Li, Hongwei
    Xu, Haiyue
    Zhao, Chunjiang
    Sulaiman, Yiming
    Wu, Changxin
    ELECTROPHORESIS, 2011, 32 (3-4) : 394 - 397
  • [4] A new, fast, and simple DNA extraction method for HLA and VNTR genotyping by PCR amplification
    Planelles, D
    Llopis, F
    Puig, N
    Montoro, JA
    JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1996, 10 (03) : 125 - 128
  • [5] A SIMPLE METHOD FOR DNA EXTRACTION FROM LEUKOCYTES FOR USE IN PCR
    LEWIN, HA
    STEWARTHAYNES, JA
    BIOTECHNIQUES, 1992, 13 (04) : 522 - &
  • [6] A rapid DNA extraction method for PCR amplification from wetland soils
    Li, J.
    Li, B.
    Zhou, Y.
    Xu, J.
    Zhao, J.
    LETTERS IN APPLIED MICROBIOLOGY, 2011, 52 (06) : 626 - 633
  • [7] Experiments in DNA extraction and PCR amplification from bighorn sheep feces: the importance of DNA extraction method
    Wehausen, JD
    Ramey, RR
    Epps, CW
    JOURNAL OF HEREDITY, 2004, 95 (06) : 503 - 509
  • [8] A simple modified method of DNA extraction from seeds for PCR amplifications
    Gupta, Ramwant
    Chandrashekar, U. S.
    Chakrabarty, S. K.
    Dadlani, M.
    INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 2012, 82 (01): : 75 - 77
  • [9] A SIMPLE AND RAPID DNA EXTRACTION PROTOCOL OF SMALL INSECTS FOR PCR AMPLIFICATION
    Tian, En-Wei
    Yu, Hui
    ENTOMOLOGICAL NEWS, 2013, 123 (04) : 303 - 310
  • [10] Development of rapid and simple method for DNA extraction from cannabis resin based on the evaluation of relative PCR amplification ability
    Yamamuro, Tadashi
    Iwata, Yuko T.
    Segawa, Hiroki
    Kuwayama, Kenji
    Tsujikawa, Kenji
    Kanamori, Tatsuyuki
    Inoue, Hiroyuki
    FORENSIC SCIENCE INTERNATIONAL, 2018, 287 : 176 - 182
12345