Simple method for DNA extraction from pancreatic juice for PCR amplification assays

被引:0
|
作者
Petra Müller
Ralf Jesnowski
Stefan Liebe
Arndt Rolfs
Matthias Löhr
机构
[1] University of Rostock,Division of Gastroenterology, Department of Medicine
[2] University of Rostock,Department of Neurology
来源
关键词
PCR; pancreatic juice; DNA; ras; DNA preparation;
D O I
暂无
中图分类号
学科分类号
摘要
Conclusion: Preparation of DNA from pancreatic juice for subsequent polymerase chain reaction (PCR) is difficult, but manageable. The protocol presented offers a simple and fast solution. This method might be applicable to other complicated samples, such as saliva, wound secretions, or stool washings.
引用
收藏
页码:39 / 43
页数:4
相关论文
共 50 条
  • [41] A SIMPLE TECHNIQUE FOR ISOLATION OF DNA SUITABLE FOR PCR AMPLIFICATION FROM CYTOGENETIC PREPARATIONS
    NUNES, MV
    DEMELO, MB
    COSTA, FF
    VARELLAGARCIA, M
    REVISTA BRASILEIRA DE GENETICA, 1995, 18 (03): : 489 - 490
  • [42] A simple DNA extraction method suitable for PCR detection of genetically modified maize
    Porcar, Manuel
    Ramos, Silvia
    Latorre, Amparo
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2007, 87 (14) : 2728 - 2731
  • [43] A rapid DNA extraction method for quantitative real-time PCR amplification from fresh water sediment
    Lu, Shimin
    Liao, Mingjun
    Zhang, Min
    Qi, Pengzhi
    Xie, Congxin
    He, Xugang
    JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT, 2012, 10 (3-4): : 1252 - 1255
  • [44] A method for total microbial DNA extraction directly meeting the requirement of PCR amplification from an Aeolian sandy soil
    Zhang, Ying
    Cao, Cheng-You
    Dongbei Daxue Xuebao/Journal of Northeastern University, 2010, 31 (11): : 1640 - 1643
  • [45] A simple and inexpensive method for genomic DNA extraction from fungi using Whatman FTA? Elute filter paper for conventional and real-time PCR assays
    Gorton, R. L.
    Vyzantiadis, T. A.
    Seaton, S.
    Kibbler, C. C.
    MYCOSES, 2009, 52 : 96 - 96
  • [46] A simple method of DNA extraction from Mycobacterium tuberculosis
    Jaber, M
    Rattan, A
    Verma, A
    Tyagi, J
    Kumar, R
    TUBERCLE AND LUNG DISEASE, 1995, 76 (06): : 578 - 581
  • [47] A Simple Method for DNA Extraction from Activated Sludge
    Singka, Dounghatai
    Kumdhitiahutsawakul, Ladapa
    Rekkriangkrai, Prasert
    Pathom-aree, Wasu
    CHIANG MAI JOURNAL OF SCIENCE, 2012, 39 (01): : 111 - 118
  • [48] A SIMPLE METHOD FOR DETERMINATION OF LIPASE ( ESTERASE ) IN PANCREATIC JUICE
    BANG, HO
    SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1965, 17 (01): : 25 - &
  • [49] A RAPID AND INEXPENSIVE METHOD FOR THE DIRECT PCR AMPLIFICATION OF DNA FROM PLANTS
    Bellstedt, Dirk U.
    Pirie, Michael D.
    Visser, J. Christiaan
    de Villiers, Margaret J.
    Gehrke, Berit
    AMERICAN JOURNAL OF BOTANY, 2010, 97 (07) : E65 - E68
  • [50] A universal DNA extraction and PCR amplification method for fungal rDNA sequence-based identification
    Romanelli, A. M.
    Fu, J.
    Herrera, M. L.
    Wickes, B. L.
    MYCOSES, 2014, 57 (10) : 612 - 622