IN-VITRO EFFECT OF 5-FLUOROURACIL, VERAPAMIL AND HYPERTHERMIA ON THE HUMAN PANCREATIC ADENOCARCINOMA CELL-LINE ASPC-1

被引：8
|
作者：
SHCHEPOTIN, IB
BURAS, RR
NAUTA, RJ
SHABAHANG, M
SOLDATENKOV, VA
EVANS, SRT
机构：
[1] GEORGETOWN UNIV,VINCENT T LOMBARDI CANC RES CTR,WASHINGTON,DC 20007
[2] GEORGETOWN UNIV,DEPT SURG,WASHINGTON,DC 20007
来源：
CHEMOTHERAPY | 1994年 / 40卷 / 05期
关键词：
PANCREATIC CANCER; 5-FLUOROURACIL; VERAPAMIL; HYPERTHERMIA;
D O I：
10.1159/000239217
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The purpose of this study was to assess the efficacy of verapamil (20 mu M) and hyperthermia (42 degrees C) as modifiers of 5-fluorouracil (5-FU) used at different concentrations, in inhibiting the growth of human pancreatic adenocarcinoma cells. Combined verapamil and hyperthermia treatment significantly decreased cell count by 63.8% when compared to the control. Verapamil drastically enhanced the growth inhibitory effect of 5-FU at all concentrations. At high-concentration 5-FU (50 mu g/ml), verapamil and hyperthermia had a synergistic growth-inhibitory effect and caused a significant decrease in cell count by 81.4% in comparison to the control. The modalities analyzed in this study require further investigation and have potential for clinical applicability to pancreatic cancer therapy in the future.
引用
收藏
页码：348 / 356
页数：9
相关论文
共 50 条
  • [41] EFFECT OF CELL-LINE ON IN-VITRO METAL-ION CYTOTOXICITY
    WATAHA, JC
    HANKS, CT
    SUN, ZL
    JOURNAL OF DENTAL RESEARCH, 1994, 73 : 294 - 294
  • [42] COMBINED EFFECT OF 5-FLUOROURACIL AND CARBOPLATIN AGAINST HUMAN GASTRIC-CANCER CELL-LINES IN-VITRO AND IN-VIVO
    SAIKAWA, Y
    KUBOTA, T
    KUO, TH
    TANINO, H
    KASE, S
    FURUKAWA, T
    WATANABE, M
    ISHIBIKI, K
    KITAJIMA, M
    HOFFMAN, RM
    ANTICANCER RESEARCH, 1994, 14 (2A) : 461 - 464
  • [43] IN-VITRO EFFECT OF 5-FLUOROURACIL ON CELLS WITH AND WITHOUT PROFICIENCY IN DNA MISMATCH REPAIR
    CARETHERS, JM
    KOI, M
    HAWN, MT
    MARRA, G
    BOLAND, CR
    GASTROENTEROLOGY, 1995, 108 (04) : A454 - A454
  • [44] MiR-3178 is Up-regulated in Pancreatic Cancer Cell Line AsPC-1 and Impacts the Malignant Behavior of Pancreatic Cancer Cells
    Yan, C. Q.
    Dong, Z.
    Xu, C.
    Duan, J. Y.
    Wang, Y. P.
    PANCREAS, 2020, 49 (10) : 1438 - 1438
  • [45] ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN LUNG ADENOCARCINOMA CELL-LINE (LC-2/AD) PRODUCING ALPHA(1)-ANTITRYPSIN IN-VITRO
    KATAOKA, H
    ITOH, H
    SEGUCHI, K
    KOONO, M
    ACTA PATHOLOGICA JAPONICA, 1993, 43 (10): : 566 - 573
  • [46] INDUCTION OF APOPTOSIS IN A PANCREATIC-CANCER CELL-LINE BY EICOSAPENTAENOIC ACID IN-VITRO
    LAI, PBS
    ROSS, JA
    FEARON, KCH
    CARTER, DC
    BRITISH JOURNAL OF SURGERY, 1995, 82 (11) : 1569 - 1569
  • [47] In-vitro effect of a combination of 5-fluorouracil (5-FU) and cisplatin (CDDP) on human gastric cancer cell lines: Timing of cisplatin treatment
    Cho H.
    Imada T.
    Oshima T.
    Shiozawa M.
    Rino Y.
    Takanashi Y.
    Gastric Cancer, 2002, 5 (1) : 43 - 46
  • [48] Synergistic effect of CGS16949A and 5-fluorouracil on a human breast cancer cell line
    Tsunoda, Y
    Shimizu, Y
    Tsunoda, A
    Shibusawa, M
    Kamiya, K
    Kusano, M
    Fukuchi, K
    EUROPEAN SURGICAL RESEARCH, 2001, 33 (03) : 232 - 236
  • [49] THE COMBINED-ACTION OF ICI-D1694, 5-FLUORO-2'-DEOXYURIDINE AND 5-FLUOROURACIL IN INHIBITING THE GROWTH OF A HUMAN RENAL-CELL CARCINOMA CELL-LINE (RPMI-SE) IN-VITRO
    GUIMARAES, MA
    GRECO, WR
    SLOCUM, HK
    HUBEN, RP
    RUSTUM, YM
    INTERNATIONAL JOURNAL OF ONCOLOGY, 1994, 4 (01) : 137 - 141
  • [50] Effect of green tea on pharmacokinetics of 5-fluorouracil in rats and pharmacodynamics in human cell lines in vitro
    Qiao, Jinping
    Gu, Chenxin
    Shang, Weihu
    Du, Jinglei
    Yin, Wei
    Zhu, Meilin
    Wang, Wei
    Han, Mei
    Lu, Weidong
    FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (06) : 1410 - 1415
12345