INCREASE OF CALLUS AND EMBRYOID PRODUCTION FROM HYPOCOTYL PROTOPLASTS OF SUNFLOWER (HELIANTHUS-ANNUUS L) BY CULTURE IN MICRODROPS

被引：3
|
作者：
SAMAJ, J
OKOLOT, A
BOBAK, M
GLEBA, Y
机构：
[1] Department of Plant Physiology, Comenius University, Bratislava, 842 15
[2] Institute of Cell Biology and Engineering, Kiev GSP 22, 252 650
来源：
BIOLOGIA PLANTARUM | 1994年 / 36卷 / 02期
关键词：
D O I：
10.1007/BF02921084
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77 % final plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins and cytokinins grew on agar solidified media without growth regulators.
引用
收藏
页码：183 / 190
页数：8
相关论文
共 50 条
  • [21] PLANTLET FORMATION FROM CALLUS AND SHOOT-TIP CULTURE OF HELIANTHUS-ANNUUS (L)
    LUPI, MC
    BENNICI, A
    LOCCI, F
    GENNAI, D
    PLANT CELL TISSUE AND ORGAN CULTURE, 1987, 11 (01) : 47 - 55
  • [22] SOMATIC HYBRIDS OF SUNFLOWER (HELIANTHUS-ANNUUS L) IDENTIFIED AT THE CALLUS STAGE BY ISOENZYME ANALYSIS
    BARTH, S
    VOESTE, D
    SCHNABL, H
    BOTANICA ACTA, 1993, 106 (01): : 100 - 102
  • [23] ORIGIN AND FATE OF ANEUSOMATY IN SUNFLOWER (HELIANTHUS-ANNUUS L)
    CREMONINI, R
    CAVALLINI, A
    PLANT BREEDING, 1986, 97 (01) : 89 - 92
  • [24] THE MECHANICS OF ANCHORAGE IN SEEDLINGS OF SUNFLOWER, HELIANTHUS-ANNUUS L
    ENNOS, AR
    NEW PHYTOLOGIST, 1989, 113 (02) : 185 - 192
  • [25] INDUCTION OF SOMATIC EMBRYO DEVELOPMENT BY LIQUID CULTURE IN SUNFLOWER (HELIANTHUS-ANNUUS L)
    PRADO, E
    BERVILLE, A
    PLANT SCIENCE, 1990, 67 (01) : 73 - 82
  • [26] AFLATOXIN PRODUCTION IN SUNFLOWER (HELIANTHUS-ANNUUS) SEED VARIETIES
    NAGARAJAN, V
    BHAT, RV
    TULPULE, PG
    CURRENT SCIENCE, 1974, 43 (19): : 603 - 604
  • [27] VARIATION IN SALINITY TOLERANCE IN SUNFLOWER (HELIANTHUS-ANNUUS L)
    ASHRAF, M
    TUFAIL, M
    JOURNAL OF AGRONOMY AND CROP SCIENCE-ZEITSCHRIFT FUR ACKER UND PFLANZENBAU, 1995, 174 (05): : 351 - 362
  • [28] PROTEOLYTIC ACTIVITY IN SUNFLOWER SEEDS (HELIANTHUS-ANNUUS L)
    WALDE, P
    LUISI, PL
    PALMIERI, S
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1984, 32 (02) : 322 - 329
  • [29] PHYTOSTEROL COMPOSITION IN THE POLLEN OF SUNFLOWER, HELIANTHUS-ANNUUS L
    TAKATSUTO, S
    OMOTE, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (12): : 3363 - 3364
  • [30] A regeneration protocol for sunflower (Helianthus annuus L) protoplasts
    Wingender, R
    Henn, HJ
    Barth, S
    Voeste, D
    Machlab, H
    Schnabl, H
    PLANT CELL REPORTS, 1996, 15 (10) : 742 - 745
12345