INCREASE OF CALLUS AND EMBRYOID PRODUCTION FROM HYPOCOTYL PROTOPLASTS OF SUNFLOWER (HELIANTHUS-ANNUUS L) BY CULTURE IN MICRODROPS

被引：3
|
作者：
SAMAJ, J
OKOLOT, A
BOBAK, M
GLEBA, Y
机构：
[1] Department of Plant Physiology, Comenius University, Bratislava, 842 15
[2] Institute of Cell Biology and Engineering, Kiev GSP 22, 252 650
来源：
BIOLOGIA PLANTARUM | 1994年 / 36卷 / 02期
关键词：
D O I：
10.1007/BF02921084
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77 % final plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins and cytokinins grew on agar solidified media without growth regulators.
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页码：183 / 190
页数：8
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