SEPARATION OF SECONDARY ALCOHOL ENANTIOMERS USING SUPERCRITICAL FLUID CHROMATOGRAPHY

Secondary alcohol enantiomers were separated using pre-column derivatization with (R)-(-)-1-(1-naphthyl)ethyl isocyanate and supercritical fluid chromatography. The chromatographic properties of the derivatized alcohols on three kinds of archiral stationary phases were investigated in this study. The selectivity of the two enantiomers depended on the properties of the stationary phase more than those of the mobile phase. The resolution properties depended on the species of secondary alcohols. The enantiomers of secondary alcohols with a longer carbon chain were better resolved, and the resolution became poor as the hydroxyl group was located towards the center of the carbon chain.