Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

被引：81
|
作者：
Accardo, Angelo [1 ]
Blatche, Marie-Charline [1 ]
Courson, Remi [1 ]
Loubinoux, Isabelle [2 ]
Vieu, Christophe [1 ,3 ]
Malaquin, Laurent [1 ]
机构：
[1] Univ Toulouse, CNRS, LAAS, F-31400 Toulouse, France
[2] Univ Toulouse, INSERM, Toulouse NeuroImaging Ctr, ToNIC, F-31024 Ups, France
[3] Univ Toulouse, INSA, F-31400 Toulouse, France
来源：
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS | 2018年 / 4卷 / 02期
基金：
欧盟地平线“2020”;
关键词：
direct laser writing; cell scaffold; 3D fabrication; PEGDA hydrogel; neuro2A cell; multi-photon confocal imaging;
D O I：
10.1088/2057-1976/aaab93
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of ss-tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.
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页数：8
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