Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

被引：81

作者：

Accardo, Angelo ^{[1
]}

Blatche, Marie-Charline ^{[1
]}

Courson, Remi ^{[1
]}

Loubinoux, Isabelle ^{[2
]}

Vieu, Christophe ^{[1
,3
]}

Malaquin, Laurent ^{[1
]}

机构：

[1] Univ Toulouse, CNRS, LAAS, F-31400 Toulouse, France

[2] Univ Toulouse, INSERM, Toulouse NeuroImaging Ctr, ToNIC, F-31024 Ups, France

[3] Univ Toulouse, INSA, F-31400 Toulouse, France

来源：

BIOMEDICAL PHYSICS & ENGINEERING EXPRESS | 2018年 / 4卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

direct laser writing; cell scaffold; 3D fabrication; PEGDA hydrogel; neuro2A cell; multi-photon confocal imaging;

D O I：

10.1088/2057-1976/aaab93

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of ss-tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.

引用

页数：8

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