CHARACTERISTICS AND SUBCELLULAR-LOCALIZATION OF PRISTANOYL-COA SYNTHETASE IN RAT-LIVER

被引:32
|
作者
WANDERS, RJA
DENIS, S
VANROERMUND, CWT
JAKOBS, C
TENBRINK, HJ
机构
[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT CLIN BIOCHEM FO226,1105 AZ AMSTERDAM,NETHERLANDS
[2] FREE UNIV AMSTERDAM,DEPT PEDIAT,1007 MC AMSTERDAM,NETHERLANDS
关键词
PRISTANIC ACID; PHYTANIC ACID; PEROXISOME; PEROXISOMAL DISORDER; FATTY ACID OXIDATION; (RAT LIVER);
D O I
10.1016/0005-2760(92)90056-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the activation of pristanic acid to its CoA-ester in rat liver. The results show that peroxisomes, mitochondria as well as microsomes contain pristanoyl-CoA synthetase activity. On the basis of competition experiments and immunoprecipitation studies using antibodies raised against rat liver microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3) we conclude that pristanic acid is activated by the same enzyme which activates long-chain fatty acids, i.e., long-chain fatty acyl-CoA synthetase.
引用
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页码:274 / 279
页数:6
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