THERMODYNAMIC AND KINETIC ANALYSES OF DNA TRIPLEX FORMATION - APPLICATION OF FILTER-BINDING ASSAY

被引：14

作者：

SARAI, A ^{[1
]}

SUGIURA, S ^{[1
]}

TORIGOE, H ^{[1
]}

SHINDO, H ^{[1
]}

机构：

[1] TOKYO COLL PHARM, HACHIOJI, TOKYO 19203, JAPAN

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1993年 / 11卷 / 02期

关键词：

D O I：

10.1080/07391102.1993.10508724

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple and efficient method for studying equilibrium thermodynamics and kinetics of DNA triplex formation, which utilizes a filter-binding procedure. The application of this method to the triplex formation between a double-stranded homopurine-homopyrimidine and a single-stranded homopyrimidine oligonucleotides has demonstrated its ability in the quantitative estimation of equilibrium binding constants and rate constants under various conditions. Thus, this simple method can serve as a powerful tool for the systematic analysis of sequence and environmental effects on the equilibrium and kinetic quantities in the triplex formation.

引用

页码：245 / 252

页数：8

共 50 条

[41] Considering the oligonucleotide secondary structures in thermodynamic and kinetic analysis of DNA duplex formation
A. A. Lomzov
D. V. Pyshnyi
Biophysics, 2012, 57 (1) : 19 - 34
[42] Kinetic studies of Fos•Jun•DNA complex formation:: DNA binding prior to dimerization
Kohler, JJ
Schepartz, A
BIOCHEMISTRY, 2001, 40 (01) : 130 - 142
[43] Effect of a triplex-binding ligand on triple helix formation at a site within a natural DNA fragment
Brown, PM
Drabble, A
Fox, KR
BIOCHEMICAL JOURNAL, 1996, 314 : 427 - 432
[44] Characterization of the DNA-binding activity of HIV-1 integrase using a filter binding assay
Haugan, IR
Nilsen, BM
Worland, S
Olsen, L
Helland, DE
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 217 (03) : 802 - 810
[45] Kinetic and thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA
Inomata, Kosuke
Ohki, Izuru
Tochio, Hidehito
Fujiwara, Kenichiro
Hiroaki, Hidekazu
Shirakawat, Masahiro
BIOCHEMISTRY, 2008, 47 (10) : 3266 - 3271
[46] A new analytical scale DNA affinity binding assay for analyses of specific protein-DNA interactions
Kumar, NV
Bernstein, LR
ANALYTICAL BIOCHEMISTRY, 2001, 299 (02) : 203 - 210
[47] Kinetic study of the binding of triplex-forming oligonucleotides containing partial cationic modifications to double-stranded DNA
Hari, Yoshiyuki
Ijitsu, Shin
Akabane-Nakata, Masaaki
Yoshida, Takuya
Obika, Satoshi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2014, 24 (14) : 3046 - 3049
[48] TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses
Vuori, Kristiina A.
Ahlskog, Johanna K.
Sistonen, Lea
Nikinmaa, Mikko
FEBS JOURNAL, 2009, 276 (24) : 7366 - 7374
[49] Unraveling the mechanism of catalytic reactions through combined kinetic and thermodynamic analyses: Application to the condensation of ethanol
Meunier, Frederic C.
Scalbert, Julien
Thibault-Starzyk, Frederic
COMPTES RENDUS CHIMIE, 2015, 18 (03) : 345 - 350
[50] 7-Aminoactinomycin Binding to DNA Sequences Lacking GpC Sites: A Thermodynamic and Kinetic Study
Biver, Tarita
Venturini, Marcella
Jares-Erijman, Elizabeth A.
Jovin, Thomas M.
Secco, Fernando
BIOCHEMISTRY, 2009, 48 (01) : 173 - 179

← 1 2 3 4 5 →