THERMODYNAMIC AND KINETIC ANALYSES OF DNA TRIPLEX FORMATION - APPLICATION OF FILTER-BINDING ASSAY

被引：14
|
作者：
SARAI, A [1 ]
SUGIURA, S [1 ]
TORIGOE, H [1 ]
SHINDO, H [1 ]
机构：
[1] TOKYO COLL PHARM, HACHIOJI, TOKYO 19203, JAPAN
来源：
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1993年 / 11卷 / 02期
关键词：
D O I：
10.1080/07391102.1993.10508724
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a simple and efficient method for studying equilibrium thermodynamics and kinetics of DNA triplex formation, which utilizes a filter-binding procedure. The application of this method to the triplex formation between a double-stranded homopurine-homopyrimidine and a single-stranded homopyrimidine oligonucleotides has demonstrated its ability in the quantitative estimation of equilibrium binding constants and rate constants under various conditions. Thus, this simple method can serve as a powerful tool for the systematic analysis of sequence and environmental effects on the equilibrium and kinetic quantities in the triplex formation.
引用
收藏
页码：245 / 252
页数：8
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