PURIFICATION OF YEAST GLUCOSE TOLERANCE FACTOR AND ITS EFFECT ON INTRACORPORAL GLUCOSE CONVERSION IN PHYSICAL ACTIVITY

Objective: this paper was aimed to study the separation and purification of yeast glucose tolerance factor (GTF) and its effect on intracorporal glucose conversion of body builders. Methods: Yeast high-yield GTF was adopted in order to improve the detection sensitivity of chromium; in separation and purification process, the method high performance liquid chromatography-inductively coupled plasma-mass spectrometry/atomic emission spectrometry (HPLC-ICPMS/AES) was applied in microanalysis of the distribution of GTF; according to the analysis results, appropriate separation technique was selected for batch separation and purification; by comparing the blood indexes of fed mice, intracorporal glucose conversion of body builders could be deduced. Results: Chromium/protein ratio of Peak 1 was much lower than that of Peak 2; Peak1 was micromolecular chromium-binding protein, while Peak 2 was micromolecular chromium-binding protein. N-terminal amino acid sequence analysis results of peak PP1 showed that amino acids in peak PP1 were probably connected with chromium by coordination bonds instead of peptide bonds. In addition, triglyceride and cholesterol level in blood decreased. Conclusion: Chromium-enriched yeast has an improvement effect on diabetes to some degree.