EFFECT OF EXTRACTS OF SOME VEGETABLES ON PROLIFERATION AND ANTIBODY SECRETION OF HUMAN-HUMAN HYBRIDOMA CELL-LINES CULTURED IN SERUM-FREE MEDIUM

The effect of extracts of some vegetables on proliferation and immunoglobulin (IgM) secretion of human-human hybridoma cell lines (HB 4 C 5 and SI 102) in a serum-free medium was examined. The aqueous dialyzates of broccoli, carrot, Chinese chive, chingentsuai, kinusayaendo, oyster fungus, spinach and sweet potato promoted the proliferation of HB 4 C 5 and SI 102 cells. The relative proliferation-promoting activity of these dialyzates was about 20 approximately 40%. A similar proliferation-promoting activity was also found in the ethanol- and dimethyl sulfoxide (DMSO)-soluble extracts of oyster fungus, kinusayaendo, Chinese chive and spinach. The aqueous dialyzates of oyster fungus, Chinese chive, sweet potato and carrot, the ethanol-soluble extracts of oyster fungus, kinusayaendo, and the DMSO-soluble extracts of oyster fungus, Chinese chive, kinusayaendo and nigauri promoted the IgM secretion of HB 4 C 5 cells.