Lessons Learned Moving From a Rapid Immunoassay to a Molecular Platform for Respiratory Viral Testing During Influenza Season

被引：1
|
作者：
Magauran, Claire E. [1 ]
Yen-Lieberman, Belinda [1 ]
Dhar, Sorabh [1 ]
Schindler, Susan [1 ]
Starkey, Colleen [1 ]
Gordon, Steven M. [1 ]
机构：
[1] Cleveland Clin, Cleveland, OH 44195 USA
来源：
INFECTIOUS DISEASES IN CLINICAL PRACTICE | 2007年 / 15卷 / 01期
关键词：
D O I：
10.1097/01.idc.0000239714.57886.75
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Accurate, rapid detection and reporting of respiratory viral illness is a challenge for the laboratory during influenza season. We replaced direct fluorescent antibody (DFA) and viral cultures with rapid enzyme immunoassay (EIA) testing for influenza A (Flu A), Flu B, and respiratory syncytial virus (RSV) during the 2004-2005 influenza season. Negative, rapid EIA tests were confirmed by culture or DFA. Results were disappointing because EIA missed 83% (19/23) of DFA (+) RSV tests and 64% (56/88) of culture (+) influenza tests. We moved to a molecular platform using a one-step real-time reverse transcriptase-polymerase chain reaction assay for the detection of Flu A, Flu B, and RSV for the 2005-2006 influenza season. Of 2094 samples tested, 12.5% (261/2094) were positive for Flu A, 3.9% (82/2094) for Flu B, and 13.3% (277/2094) for RSV. We found real-time PCR to be an acceptable platform for respiratory virus testing during the influenza season.
引用
收藏
页码：22 / 25
页数：4
相关论文
共 50 条
  • [41] Droplet precautions on-site (DroPS) during the influenza season 2018/2019: a possible alternative to single room isolation for respiratory viral infections
    Birrer, Michele
    Perrig, Martin
    Hobi, Fabienne
    Gfeller, Christina
    Atkinson, Andrew
    Egger, Martin
    Bartholdi, Corinne
    Aujesky, Drahomir
    Marschall, Jonas
    Sommerstein, Rami
    ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 2022, 11 (01)
  • [42] Clinical and molecular epidemiology of influenza viruses from Romanian patients hospitalized during the 2019/20 season
    Miron, Victor Daniel
    Banica, Leontina
    Sandulescu, Oana
    Paraschiv, Simona
    Surleac, Marius
    Florea, Dragos
    Vlaicu, Ovidiu
    Milu, Petre
    Streinu-Cercel, Anca
    Bilasco, Anuta
    Otelea, Dan
    Pitigoi, Daniela
    Streinu-Cercel, Adrian
    Draganescu, Anca Cristina
    PLOS ONE, 2021, 16 (11):
  • [43] Optimization of Thyroid Samples for Molecular Testing: Lessons Learned from Indeterminate Thyroid Fine Needle Aspirations with Sub-Optimal Cytology and Molecular Testing Results
    Lynn, Terrance
    Ding, Yi
    Alvi, Madiha
    Zelonis, Michele
    Monaco, Sara
    LABORATORY INVESTIGATION, 2021, 101 (SUPPL 1) : 247 - 248
  • [44] Optimization of Thyroid Samples for Molecular Testing: Lessons Learned from Indeterminate Thyroid Fine Needle Aspirations with Sub-Optimal Cytology and Molecular Testing Results
    Lynn, Terrance
    Ding, Yi
    Alvi, Madiha
    Zelonis, Michele
    Monaco, Sara
    MODERN PATHOLOGY, 2021, 34 (SUPPL 2) : 247 - 248
  • [45] Catching the Flu: Clinical Implications of Two Different Molecular Testing Methods Used During the 2013-2014 Influenza Season
    Csikesz, Nicholas
    Chen, Sonja
    Smith, Jacob
    Dickenson, Roberta
    Chapin, Kimberle
    Carino, Gerardo
    CHEST, 2015, 148 (04)
  • [46] When the COVID-19 Pandemic Surges during Influenza Season: Lessons Learnt from the Sentinel Laboratory-Based Surveillance of Influenza-Like Illness in Lombardy during the 2019-2020 Season
    Galli, Cristina
    Pellegrinelli, Laura
    Bubba, Laura
    Primache, Valeria
    Anselmi, Giovanni
    Delbue, Serena
    Signorini, Lucia
    Binda, Sandro
    Cereda, Danilo
    Gramegna, Maria
    Pariani, Elena
    VIRUSES-BASEL, 2021, 13 (04):
  • [47] Increasing hepatitis C testing uptake with point of care testing from the needle and syringe exchange desk - lessons learned from the Rapid-EC Feasibility Study
    Williams, B.
    Pedrana, A.
    Howell, J.
    Doyle, J.
    Thompson, A.
    Bramwell, F.
    Membrey, D.
    McPherson, M.
    Layton, C.
    Draper, B.
    Roney, J.
    Latham, N.
    Hellard, M.
    JOURNAL OF VIRAL HEPATITIS, 2018, 25 : 28 - 29
  • [48] Discordant results between biochemical and molecular transthyretin assays: lessons learned from a unique testing algorithm at the Mayo Clinic
    HONEY V. REDDI
    BRITTANY C. THOMAS
    KURT S. WILLKOMM
    MATTHEW J. FERBER
    KANDELARIA M. RUMILLA
    KIMIYO M. RAYMOND
    JOHN F. O’BRIEN
    W. EDWARD HIGHSMITH
    Journal of Genetics, 2013, 92 : 599 - 604
  • [49] Molecular testing for Fragile X Syndrome: Lessons learned from 119,232 tests performed in a clinical laboratory
    Strom, Charles M.
    Crossley, Beryl
    Redman, Joy B.
    Buller, Arlene
    Quan, Franklin
    Peng, Mei
    McGinnis, Matthew
    Fenwick, Raymond G., Jr.
    Sun, Weimin
    GENETICS IN MEDICINE, 2007, 9 (01) : 46 - 51
  • [50] Discordant results between biochemical and molecular transthyretin assays: lessons learned from a unique testing algorithm at the Mayo Clinic
    Reddi, Honey V.
    Thomas, Brittany C.
    Willkomm, Kurt S.
    Ferber, Matthew J.
    Rumilla, Kandelaria M.
    Raymond, Kimiyo M.
    O'Brien, John F.
    Highsmith, W. Edward
    JOURNAL OF GENETICS, 2013, 92 (03) : 599 - 604
12345