Lessons Learned Moving From a Rapid Immunoassay to a Molecular Platform for Respiratory Viral Testing During Influenza Season

被引：1
|
作者：
Magauran, Claire E. [1 ]
Yen-Lieberman, Belinda [1 ]
Dhar, Sorabh [1 ]
Schindler, Susan [1 ]
Starkey, Colleen [1 ]
Gordon, Steven M. [1 ]
机构：
[1] Cleveland Clin, Cleveland, OH 44195 USA
来源：
INFECTIOUS DISEASES IN CLINICAL PRACTICE | 2007年 / 15卷 / 01期
关键词：
D O I：
10.1097/01.idc.0000239714.57886.75
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Accurate, rapid detection and reporting of respiratory viral illness is a challenge for the laboratory during influenza season. We replaced direct fluorescent antibody (DFA) and viral cultures with rapid enzyme immunoassay (EIA) testing for influenza A (Flu A), Flu B, and respiratory syncytial virus (RSV) during the 2004-2005 influenza season. Negative, rapid EIA tests were confirmed by culture or DFA. Results were disappointing because EIA missed 83% (19/23) of DFA (+) RSV tests and 64% (56/88) of culture (+) influenza tests. We moved to a molecular platform using a one-step real-time reverse transcriptase-polymerase chain reaction assay for the detection of Flu A, Flu B, and RSV for the 2005-2006 influenza season. Of 2094 samples tested, 12.5% (261/2094) were positive for Flu A, 3.9% (82/2094) for Flu B, and 13.3% (277/2094) for RSV. We found real-time PCR to be an acceptable platform for respiratory virus testing during the influenza season.
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页码：22 / 25
页数：4
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