A METHOD FOR SPATIOTEMPORAL (4-D) DATA REPRESENTATION IN CONFOCAL MICROSCOPY - APPLICATION TO NEUROANATOMICAL PLASTICITY

被引：11

作者：

KRIETE, A ^{[1
]}

WAGNER, HJ ^{[1
]}

机构：

[1] UNIV MARBURG,INST ANAT & ZELLBIOL,W-3550 MARBURG,GERMANY

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1993年 / 169卷

关键词：

CONFOCAL MICROSCOPY; RETINA; 3-DIMENSIONAL RECONSTRUCTION; 4-DIMENSIONAL VISUALIZATION; NEUROANATOMY; SYNAPTIC PLASTICITY;

D O I：

10.1111/j.1365-2818.1993.tb03275.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

This paper describes a new method for data representation and visualization in four dimensions (three dimensions plus time). Sequential volumes, exhibiting morphological activity, are acquired non-invasively with a confocal scanning laser microscope, where each data set corresponds to a time sample. A pipelined processing includes packing of volumes and specific volume rendering techniques. Subsequent processing in HIS (hue, intensity, saturation) colour space combines functional, packed images with shaded three-dimensional views. As a result, even subtle changes in morphology become visible and computational time is saved. Experimental findings obtained from investigations of synaptic plasticity in cultured retinal tissue are reported.

引用

页码：27 / 31

页数：5

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