Bacteriological Study of Pigmented Bacteria and Molecular Study Based for 16s rRNA Gene

Periodontitis is an infectious disease leading to the damage of periodontium. The etiology of this disease is microorganisms deposits play an essential role in the pathogenesis. The study aimed to isolation and to identification of P. gingivalis, using The PCR technique based on 16s rRNA gene to confirm of identifications. The ability of this bacterium to adhere to epithelial cells of oral cavity and evaluating the effect of some extracts on biofilm formation. Eighty clinical sample was collected from subgingival plaque of dental of patients with (50) chronic and (30) aggressive periodontitis. The patient was admitted to Babylon University (teaching Hospital of college of Dentistry). The samples of plaque were subjected to different methods for identification of P. gingivalis. It was found that twenty two of P. gingivalis isolates were recovered. Eighteen isolates obtained from (CP) & four isolates from (AP). Twelve isolates of P. gingivalis from twenty two were detected by method of molecular focusing on the role of 16s rRNA gene for this microorganism. Ten species of bacterial isolates was isolated from chronic periodontitis, and two bacterial isolates were isolated from aggressive periodontitis subgingival plaques. All bacterial isolates were investigated to detect Attachment of P. gingivalis to epithelial cells oral cavity. The result showed that all isolates have ability to attach to epithelial cells oral cavity. The study also detected the ability of the bacteria for production of gingipain; it was found that all tested isolates were positive for this enzyme at a rate 100%. In addition, biofilm formations were tested in the semi quantitative of test of microtiter plate. The results of this study revealed that the isolates were biofilm former, high and moderate biofilm formation mode were accounted for (50%) while there are no isolates that express non biofilm formation. The study was also evaluating the effect of some extracts on biofilm formation. The higher effects for Alum of the bacteria were showed followed by Clove, in contrast to lowest effect of C. rotundus. P. gingivalis were the well-recognized potential period on to pathogen. The PCR technique based of 16s rRNA gene is sensitive and bacterial culture being the gold standard of the growth and identification of P. gingivalis. all isolates able to produce biofilm. Alum, Clove, and C. rotundas have an effect on P. Gingivalis biofilm.