SELECTION OF MUTANTS OF ASPERGILLUS-NIGER SHOWING ENHANCED PRODUCTIVITY OF CITRIC-ACID FROM STARCH IN SHAKING CULTURE

被引：3
|
作者：
RUGSASEEL, S [1 ]
KIRIMURA, K [1 ]
USAMI, S [1 ]
机构：
[1] WASEDA UNIV,SCH SCI & ENGN,DEPT APPL CHEM,TOKYO 160,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 75卷 / 03期
关键词：
D O I：
10.1016/0922-338X(93)90122-O
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Mutants with enhanced citric acid production from soluble starch were induced from Aspergillus niger WU-2223L. After UV-irradiation of a conidial suspension of strain WU-2223L, mutants were selected on modified starch-methyl red agar plates on the basis of higher amylolytic activity and acid productivity. The 8 mutants selected showed enhanced citric acid production from soluble starch in shaking culture. Among them, a representative mutant strain, 2M-43, produced 48.0 g/l of citric acid from 120 g/l of soluble starch in 9 d of cultivation in shaking culture, whereas strain WU-2223L produced 35.1 g/l. Glucoamylase activities in the culture filtrates of strains 2M-43 and WU-2223L reached maximum levels of 3.62 U/ml and 2.11 U/ml, respectively, both at 3 d of cultivation, and thereafter decreased.
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页码：226 / 228
页数：3
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