Two polyhydroxylated steroids have been isolated from the South China Sea soft coral Litophyton arboreum, and their structures were established as 24-methylcholesta-5,24(28)-diene-3 beta,7 beta,19-triol and (24E)-24-ethyl-5 alpha-cholesta-8,24(28)-diene-3 beta,12 beta,19-triol from spectral evidence.