DEVELOPMENT OF AN ACID-HYDROLYSIS METHOD WITH HIGH RECOVERIES OF TRYPTOPHAN AND CYSTEINE FOR MICROQUANTITIES OF PROTEIN

被引：23

作者：

YAMADA, H ^{[1
]}

MORIYA, H ^{[1
]}

TSUGITA, A ^{[1
]}

机构：

[1] SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT BIOCHEM,SHINJUKU KU,TOKYO 162,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90496-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High recoveries of tryptophan and cysteine were achieved by 12.5 min of hydrolysis with mercaptoethanesulfonic acid vapor. Proteins (1-100 μg) were modified by vapor-phase S-pyridylethylation before hydrolysis. The modified proteins were hydrolyzed with the vapor of 2.5 m mercaptoethanesulfonic acid at 176°C. This method promoted efficient hydrolysis of the peptide bonds in proteins and resulted in high recoveries of both tryptophan and cysteine, of 90% or greater, in addition to the other amino acids. © 1991.

引用

页码：1 / 5

页数：5

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