SEPARATION AND CHARACTERIZATION OF 25-HYDROXYVITAMIN D-3 3-SULFATE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：11
|
作者：
SHIMADA, K
MITAMURA, K
MUKOUYAMA, M
OKUMURA, T
SUGAYA, K
机构：
[1] Faculty of Pharmaceutical Sciences, Kanazawa University
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1995年 / 33卷 / 02期
关键词：
D O I：
10.1093/chromsci/33.2.82
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The separation and characterization of 25-hydroxyvitamin D3 3-sulfate in human plasma are carried out by high-performance liquid chromatography. The vitamin D sulfate fraction is obtained from a plasma specimen (three volunteers) by the combined use of a Sep-Pak C18 cartridge, for solid-phase extraction, and a lipophilic gel (piperidinohydroxypropyl Sephadex LH-20), for ion-exchange chromatography. 25-Hydroxyvitamin D3 3-sulfate is identified in the examined three specimens in two ways: its chromatographic behavior and that of its fluorescent labeled derivative using 4-[4-(6-methoxy-2-benzoxazolyl)phenyl]-1,2,4-triazoline-3,5- dione; and data obtained from the solvolysis reaction.
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页码：82 / 85
页数：4
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