INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA-COLI AS A LABEL FOR THE DETECTION OF PLANT-VIRUSES BY ELISA

被引：3
|
作者：
MIZENINA, OA
BORISOVA, OV
NOVIKOV, VK
EVTUSHENKO, OA
BAYKOV, AA
ATABEKOV, JG
机构：
[1] MV LOMONOSOV STATE UNIV,DEPT VIROL,MOSCOW 119899,USSR
[2] MV LOMONOSOV STATE UNIV,AN BELOZERSKY LAB MOLEC BIOL & BIOORGAN CHEM,MOSCOW 117234,USSR
来源：
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1991年 / 133卷 / 04期
关键词：
D O I：
10.1111/j.1439-0434.1991.tb00163.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Inorganic pyrophosphatase (PPase) from E. coli was used as label in enzyme immunoassay for the detection of different plant viruses. The use of PPase-conjugated antibodies and tetrasodium pyrophosphate as a substrate in ELISA provides some advantages in detection of plant viruses in purified preparations and "tracts from plant specimens: high sensitivity, negligible level of background reactions in control samples, bright blue-greenish colour which allows to detect the reaction visually and high stability of PPase-conjugated antibodies.
引用
收藏
页码：278 / 288
页数：11
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