HIGH-RESOLUTION FLOW-CYTOMETRY OF NUCLEAR-DNA IN HIGHER-PLANTS

被引：58
|
作者：
ULRICH, I
ULRICH, W
机构：
[1] Institut für Genetik, Universität Hohenheim, Stuttgart 70, D-W-7000
来源：
PROTOPLASMA | 1991年 / 165卷 / 1-3期
关键词：
HIGHER PLANTS; NUCLEAR DNA; NUCLEAR PROTEIN; FLOW CYTOMETRY; DAPI; SR-101;
D O I：
10.1007/BF01322292
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
High-resolution flow cytometry of nuclear DNA in higher plants has been performed from chopped plant tissues and plant protoplasts. A preparation and staining procedure with the DNA specific fluorochrome DAPI, successfully employed for precise flow cytometric DNA analysis of animal and human cells has been used in a slightly modified manner for the DNA analysis of plant cell material. High-resolution DNA histograms coefficients of variation about 1-1.5% have been obtained routinely from plant species with different DNA content. Staining of nuclei with DAPI in combination with the protein fluorochrome sulforhodamine 101 allows bi-parametric analysis of nuclear DNA and protein. The described simple and precise method might be very promising for the analysis of DNA in basic and applied cytogenetic investigations of plant cell research.
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页码：212 / 215
页数：4
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