Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus

被引：4
|
作者：
Cora-Cruz, J. J. [1 ]
Diffoot-Carlo, N. [2 ]
Sundaram, P. A. [1 ]
机构：
[1] Univ Puerto Rico, Dept Mech Engn, Mayaguez Campus, Mayaguez, PR 00680 USA
[2] Univ Puerto Rico, Dept Biol, Mayaguez Campus, Mayaguez, PR 00680 USA
来源：
DATA IN BRIEF | 2016年 / 6卷
关键词：
MC3T3-E1; Cells; Mechanical Stimulus; Focal Adhesions; Vinculin;
D O I：
10.1016/j.dib.2015.11.052
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus. (C) 2015 The Authors. Published by Elsevier Inc.
引用
收藏
页码：94 / 100
页数：7
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