WHOLE-CELL K+ CURRENTS IN ISOLATED RABBIT CORNEAL EPITHELIAL-CELLS

被引：0

作者：

TANTIRITTISAK, W ^{[1
]}

OCHI, R ^{[1
]}

KANAI, A ^{[1
]}

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT OPHTHALMOL,TOKYO 113,JAPAN

来源：

JAPANESE JOURNAL OF PHYSIOLOGY | 1991年 / 41卷 / 06期

关键词：

CORNEAL EPITHELIUM; PATCH CLAMP; POTASSIUM CURRENT; RESTING POTENTIAL;

D O I：

10.2170/jjphysiol.41.961

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Membrane currents were recorded from enzymatically isolated cells from basal layers of rabbit corneal epithelium by the whole-cell clamp technique. Pipettes contained 140.4 mm KCl and extracellular K+ concentration was varied. The membrane currents on step voltage changes were rectangular currents with some fluctuations. The fluctuations disappeared near the zero-current potential. The reversal potential in normal Tyrode's solution with 5.4 mm K+ was -57.8 +/- 6.2 mV (mean +/-S.D., n = 10). Increasing [K+]. from 5.4 to 140.4 mm shifted the reversal potentials in the positive direction with a slope of 41.0 mV/decade. Concomitant depolarization of the resting potential was observed on increasing [K+]o. The whole-cell currents were blocked by Cs+ or Ba2+. These suggest that the major current component in the corneal epithelial cells is K+.

引用

页码：961 / 967

页数：7

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