IDENTIFICATION OF MELANOCORTIN 1 RECEPTOR (MC1R) GENE BASED ON COAT COLOR OF BALI COWS OF KUPANG BY USINGTHE PCR-RFLP METHOD

被引：5

作者：

Tabun, A. C. ^{[1
]}

Hartatik, T. ^{[2
]}

Sumadi ^{[2
]}

机构：

[1] Kupang State Agicultural Polytech, Jl AdisuciptoPenfui, Kupang Ntt 1152, Indonesia

[2] Gadjah Mada Univ, Fac Anim Sci, Bulaksumur 55281, Yogyakarta, Indonesia

来源：

JOURNAL OF THE INDONESIAN TROPICAL ANIMAL AGRICULTURE | 2013年 / 38卷 / 02期

关键词：

Melanocortin; 1; Receptor; Bali cow; coat color; amplification; monomorphic;

D O I：

10.14710/jitaa.38.2.86-91

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective of this study was to identify the Melanocortin 1 Receptor (MC1R) gene in sorrel-, black-and white-Bali cows originated from Kupang-East Nusa Tenggara. Blood samples were taken from 46 cows consisted of of 17, 18 and 8 of sorrel, black and 8 heads, respectively, and 3 heads of bull. The PCR-RFLP method was performed to identify the gene. Amplification of PCR gene MC1R of Kupang's Bali Cattle (KBC) was 296 bp. The digestion by using MspI enzyme showed that there were EE genotype (169, 136 bp) in Bali bull, sorrel and white cows. Two 2 genotypes, those were EE (169, 136 bp) and Ee (296, 169, 136 bp) were found in black Bali cow. The frequency of E alele of KBC was 0.99 (99%) and e alele was 0.01 (1%). MC1R gene in KBC was monomorphic and the it can be used as the marker of the coat color in the population.

引用

页码：86 / 91

页数：6

共 50 条

[31] Variants of the melanocortin 1 receptor gene (MC1R) and P gene as indicators of the population origin of an individual
Sosuke Masui
Masato Nakatome
Ryoji Matoba
International Journal of Legal Medicine, 2009, 123 : 205 - 211
[32] Variants of the melanocortin 1 receptor gene (MC1R) and P gene as indicators of the population origin of an individual
Masui, Sosuke
Nakatome, Masato
Matoba, Ryoji
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2009, 123 (03) : 205 - 211
[33] Association of melanocortin 1 receptor gene (MC1R) polymorphisms with skin reflectance and freckles in Japanese
Kyoko Yamaguchi
Chiaki Watanabe
Akira Kawaguchi
Takehiro Sato
Izumi Naka
Misaki Shindo
Keiichi Moromizato
Kenichi Aoki
Hajime Ishida
Ryosuke Kimura
Journal of Human Genetics, 2012, 57 : 700 - 708
[34] Genetic polymorphisms in the 5'-flanking region of the melanocortin 1 receptor (MC1R) gene in foxes
Gong, Y. F.
Liu, Z. Z.
Zhang, W. X.
Feng, M. S.
Duan, L. X.
Zhu, W. J.
Liu, X. R.
Wu, J. H.
Ge, M. X.
Li, X. L.
RUSSIAN JOURNAL OF GENETICS, 2017, 53 (04) : 490 - 497
[35] Association of melanocortin 1 receptor gene (MC1R) polymorphisms with skin reflectance and freckles in Japanese
Yamaguchi, Kyoko
Watanabe, Chiaki
Kawaguchi, Akira
Sato, Takehiro
Naka, Izumi
Shindo, Misaki
Moromizato, Keiichi
Aoki, Kenichi
Ishida, Hajime
Kimura, Ryosuke
JOURNAL OF HUMAN GENETICS, 2012, 57 (11) : 700 - 708
[36] Genetic polymorphisms in the 5'-flanking region of the melanocortin 1 receptor (MC1R) gene in foxes
Y. F. Gong
Z. Z. Liu
W. X. Zhang
M. S. Feng
L. X. Duan
W. J. Zhu
X. R. Liu
J. H. Wu
M. X. Ge
X. L. Li
Russian Journal of Genetics, 2017, 53 : 490 - 497
[37] Genetics and evolution of plumage color in Crested Ibis: Analysis of the melanocortin-1 receptor (MC1R)
Yang, J.
Liu, X.
Zhang, J.
Qing, B.
Lu, B.
CELLULAR AND MOLECULAR BIOLOGY, 2015, 61 (04) : 63 - 69
[38] Evolution of the melanocortin-1 receptor gene (MC1R) in chamois (Rupicapra spp.)
Perez, Trinidad
Essler, Sabine
Palacios, Borja
Albornoz, Jesus
Dominguez, Ana
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2013, 67 (03) : 621 - 625
[39] Variants of melanocortin 1 receptor (MC1R) gene and non-melanoma skin cancer
Bastiaens, MT
Gruis, NA
ter Huurne, J
Bavinck, JNB
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1999, 113 (03) : 500 - 500
[40] Melanocortin-1 Receptor (MC1R) Gene Variants are Not Associated With Vascular Brain Alterations
Candrea, E.
Podlipnik, S.
Oleaga, L.
Puig, S.
ACTAS DERMO-SIFILIOGRAFICAS, 2023, 114 (01): : 80 - 82

← 1 2 3 4 5 →