AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS STEM SEGMENTS AND REGENERATION OF TRANSGENIC PLANTS

被引：135
|
作者：
MOORE, GA
JACONO, CC
NEIDIGH, JL
LAWRENCE, SD
CLINE, K
机构：
[1] Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, 32611, FL
来源：
PLANT CELL REPORTS | 1992年 / 11卷 / 5-6期
关键词：
CARRIZO CITRANGE; KEY LIME (C-AURANTIFOLIA); GENETIC TRANSFORMATION;
D O I：
10.1007/BF00235073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker beta-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (less-than-or-equal-to 5%) of the shoots regenerated in the presence of 100-mu-g/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
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页码：238 / 242
页数：5
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