ENZYMATIC PRODUCTION OF OLIGOSACCHARIDES FROM CORNCOB XYLAN

被引：63
|
作者：
PELLERIN, P
GOSSELIN, M
LEPOUTRE, JP
SAMAIN, E
DEBEIRE, P
机构：
[1] INRA, Station de Technologie Alimentaire, Villeneuve d'Ascq
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 08期
关键词：
CLOSTRIDIUM-THERMOLACTICUM; ENDOXYLANASE; CORNCOB XYLAN; XYLO-OLIGOSACCHARIDES; ARABINO-XYLO-OLIGO-SACCHARIDES;
D O I：
10.1016/0141-0229(91)90074-K
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An endo-1,4-beta-xylanase (E.C.3.2.1.8) fraction of high specific activity was isolated from a 180-l culture supernatant of Clostridium thermolacticum and partially purified by a one-step ion-exchange chromatography. This xylanase fraction, which contained 290 U mg-1 of protein, was used for the large-scale depolymerization of a xylan extracted from corncob meal. The oligosaccharides produced were adsorbed on charcoal and eluted stepwise with ethanol to yield mainly xylobiose, xylotriose, and arabino-xylo-oligosaccharides.
引用
收藏
页码：617 / 621
页数：5
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