The growth of sake yeast was significantly inhibited by isoamyl monofluoroacetate. This inhibition was caused by free monofluoroacetic acid, a highly toxic compound, formed from isoamyl monofluoroacetate by yeast esterase. Yeast mutants with low esterase activity were expected to become resistant to isoamyl monofluoroacetate. Fifty mutants resistant to this ester were isolated from haploid strains derived from sake yeast. Two strains which had less than 15% of the original activity of esterase were obtained. These 2 strains could be used for brewing sake like their parental strain, and produce sake which contained 1.5 to 2.0 times higher amounts of isoamyl acetate and isobutyl acetate and had an excellent fruity flavor.
机构:
Hakutsuru Sake Brewing Co Ltd, Dept Res & Dev, Higashinada Ku, Kobe, Hyogo 6580041, JapanHakutsuru Sake Brewing Co Ltd, Dept Res & Dev, Higashinada Ku, Kobe, Hyogo 6580041, Japan
机构:
Kyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, JapanKyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, Japan
Hirooka, Kiyoo
Ogita, Akira
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Osaka City Univ, Res Ctr Urban Hlth & Sports, Sumiyoshi Ku, Osaka 5588585, Japan
Osaka City Univ, Dept Bio & Geosci, Grad Sch Sci, Sumiyoshi Ku, Osaka 5588585, JapanKyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, Japan
Ogita, Akira
Fujita, Ken-Ichi
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Osaka City Univ, Dept Bio & Geosci, Grad Sch Sci, Sumiyoshi Ku, Osaka 5588585, JapanKyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, Japan
Fujita, Ken-Ichi
Yamamoto, Yoshihiro
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Kyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, JapanKyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, Japan
Yamamoto, Yoshihiro
Tanaka, Toshio
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Osaka City Univ, Dept Bio & Geosci, Grad Sch Sci, Sumiyoshi Ku, Osaka 5588585, JapanKyoto Municipal Inst Ind Technol & Culture, Shimogyo Ku, Kyoto 6008815, Japan