ADRENOMEDULLIN STIMULATES CYCLIC-AMP FORMATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS

被引：324
|
作者：
ISHIZAKA, Y [1 ]
ISHIZAKA, Y [1 ]
TANAKA, M [1 ]
KITAMURA, K [1 ]
KANGAWA, K [1 ]
MINAMINO, N [1 ]
MATSUO, H [1 ]
ETO, T [1 ]
机构：
[1] NATL CARDIOVASC CTR, RES INST, SUITA, OSAKA 565, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 01期
关键词：
D O I：
10.1006/bbrc.1994.1496
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC(50) value of human AM is 2 X 10(-8)M which is slightly higher than that of CGRP (8.5 X 10(-9)M). In a receptor binding assay for AM in rat VSMC, the binding of [I-125] AM was competitively inhibited by human AM, but not by human CGRP. Thus, AM is thought to increase intracellular cAMP in rat VSMC via its specific receptor to evoke vasodilation. (C) 1994 Academic Press, Inc.
引用
收藏
页码：642 / 646
页数：5
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