NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF EMPAGLIFLOZIN IN HUMAN PLASMA

A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50: 50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm x 4.6 mm, 5 mu m) with photodiode arrays (PDA) detection at 270 nm. The bioanalytical procedure involves deproteination of plasma with 30% ethyl acetate and liquid-liquid extraction process. Chromatogram showed a peak of empagliflozin at retention time of 8.898 min. The correlation coefficient (r(2)) was found to be 0.999 in the concentration range of 50-150 mu g/ml. No interference peak was observed in blank plasma samples at the retention time of empagliflozin. The percentage of relative recovery and coefficient of variation (CV %) values of precision and accuracy were within the acceptable limits. The method proved simple, cost effective and sensitive for estimation of empagliflozin in human plasma.