HIGH-FREQUENCY SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION FROM HYPOCOTYL PROTOPLAST CULTURES OF BRASSICA-NAPUS

被引：8
|
作者：
PARIHAR, DS [1 ]
MAHESHWARI, SC [1 ]
KHURANA, P [1 ]
机构：
[1] UNIV DELHI,DEPT PLANT MOLEC BIOL,NEW DELHI 110021,INDIA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 42卷 / 01期
关键词：
HYPOCOTYL PROTOPLAST; BRASSICA NAPUS; SOMATIC EMBRYOGENESIS;
D O I：
10.1007/BF00037689
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple protocol has been developed for high frequency protoplast regeneration via somatic embryogenesis in B. napus. Protoplasts isolated from hypocotyl tissue of 8-12 day old seedlings of Brassica napus ISN706 (AACC) when cultured in KM(A) medium resulted in divisions with a, frequency ranging from 30-35%. Regeneration of plantlets was possible by both organogenesis and embryogenesis. Nearly 80% of the call transferred on to MS medium supplemented with 5.0 mg 1(-1) 2iP, 0.1 mg 1(-1) NAA, 0.001 mg 1(-1) GA(3), 0.5 g 1(-1) PVP and 0.5 g 1(-1) MES displayed somatic embryogenesis. The somatic embryos developed into normal plantlets, and also displayed secondary, repetitive embryogenesis.
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页码：113 / 115
页数：3
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