APOPTOSIS INDUCED BY PROTEIN-KINASE-C INHIBITION IN A NEUROBLASTOMA CELL-LINE

被引：0
|
作者：
BEHRENS, MM [1 ]
MARTINEZ, JL [1 ]
MORATILLA, C [1 ]
RENART, J [1 ]
机构：
[1] UNIV AUTONOMA MADRID, DEPT BIOCHEM, CSIC, INST INVEST BIOMED, E-28029 MADRID, SPAIN
来源：
CELL GROWTH & DIFFERENTIATION | 1995年 / 6卷 / 11期
关键词：
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The protein kinase C inhibitor bisindolylmaleimide GF109203X has a dual effect on the behavior of tire neuroblastoma cell line Neuro-2A; when the inhibitor is added in conditions that induce differentiation (absence of serum), neurite outgrowth is potentiated in a dose-dependent manner. However, if the inhibitor is added in growth-promoting conditions (presence of serum), programmed cell death (apoptosis) is induced, as assessed by internucleosomal DNA cleavage and specific immunoassays. This effect is also seen with other specific protein kinase C inhibitors. Bcl2 gene overexpression protects Neuro-2A cells from apoptosis, as has been found in other systems. We also show that calpain I, a neutral Ca2+-activated proteinase, participates in this apoptotic pathway. Our results point to a key role of protein kinase C in the regulation of growth and differentiation in Neuro-2A cells.
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页码：1375 / 1380
页数：6
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