APOPTOSIS INDUCED BY PROTEIN-KINASE-C INHIBITION IN A NEUROBLASTOMA CELL-LINE

被引：0

作者：

BEHRENS, MM ^{[1
]}

MARTINEZ, JL ^{[1
]}

MORATILLA, C ^{[1
]}

RENART, J ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID, DEPT BIOCHEM, CSIC, INST INVEST BIOMED, E-28029 MADRID, SPAIN

来源：

CELL GROWTH & DIFFERENTIATION | 1995年 / 6卷 / 11期

关键词：

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The protein kinase C inhibitor bisindolylmaleimide GF109203X has a dual effect on the behavior of tire neuroblastoma cell line Neuro-2A; when the inhibitor is added in conditions that induce differentiation (absence of serum), neurite outgrowth is potentiated in a dose-dependent manner. However, if the inhibitor is added in growth-promoting conditions (presence of serum), programmed cell death (apoptosis) is induced, as assessed by internucleosomal DNA cleavage and specific immunoassays. This effect is also seen with other specific protein kinase C inhibitors. Bcl2 gene overexpression protects Neuro-2A cells from apoptosis, as has been found in other systems. We also show that calpain I, a neutral Ca2+-activated proteinase, participates in this apoptotic pathway. Our results point to a key role of protein kinase C in the regulation of growth and differentiation in Neuro-2A cells.

引用

页码：1375 / 1380

页数：6

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