PROTEASE-CATALYSED PEPTIDE SYNTHESIS IN SOLVENT-FREE SYSTEM

被引：0
|
作者：
Cerovsky, Vaclav [1 ]
机构：
[1] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic
来源：
BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 02期
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D O I：
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中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method of enzymatic peptide synthesis without any liquid added has been proposed. The method is based on the admixing of N-acylamino acid (peptide) esters and nucleophiles (amides or tert.-butylesters of amino acids or peptides, peptides) with various proteolytic enzymes such as alpha-chymotrypsin, trypsin, proteinase K, subtilisin, elastase and papain in the presence of Na2CO3 center dot 10H(2)O. In most instances, acylating components were completely converted within a few hours giving satisfactory yields of desired peptide products.
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页码：155 / 160
页数：6
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