ISOLATION AND SOME PROPERTIES OF EXTRACELLULAR RIBONUCLEASE FROM TRICHODERMA-HARZIANUM

被引：5
|
作者：
VASILEVATONKOVA, ES
机构：
[1] Institute of Microbiology, Bulgarian Academy of Sciences, Sofia
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1993年 / 15卷 / 08期
关键词：
RIBONUCLEASE; TRICHODERMA; ISOLATION;
D O I：
10.1016/0141-0229(93)90070-I
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple and rapid procedure for isolation of a highly purified ribonuclease from the culture filtrate of Trichoderma harzianum was described. The scheme included precipitation with ammonium sulfate followed by ion-exchange chromatography on DEAE-cellulose and CM-cellulose. The molecular weight of the RNase was found to be approximately 12,000 daltons by SDS-PAGE and gel filtration. The pH optimum for the enzyme was at 7.8 and maximum activity was obtained at 40-50-degrees-C.
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页码：687 / 690
页数：4
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