A SPECIFIC OLIGONUCLEOTIDE PROBE BASED ON 5S RIBOSOMAL-RNA SEQUENCES FOR IDENTIFICATION OF VIBRIO-ANGUILLARUM AND VIBRIO-ORDALII

被引：5

作者：

ITO, H ^{[1
]}

UCHIDA, I ^{[1
]}

SEKIZAKI, T ^{[1
]}

TERAKADO, N ^{[1
]}

机构：

[1] KOURIYAMA LIVESTOCK HYG SERV CTR,KORIYAMA,FUKUSHIMA 963,JAPAN

来源：

VETERINARY MICROBIOLOGY | 1995年 / 43卷 / 2-3期

关键词：

V-ANGUILLARUM; VIBRIO-ORDALII; IDENTIFICATION; 5S RIBOSOMAL-RNA; OLIGONUCLEOTIDE PROBE;

D O I：

10.1016/0378-1135(94)00086-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An oligonucleotide DNA probe based on 5S rRNA sequence data was constructed for the identification of the fish pathogens, Vibrio anguillarum and Vibrio ordalii. Specificity of the probe was tested in a colony blot hybridization assay. The respective probe was found to be specific for both V. anguillarum and V. ordalii. No cross hybridization was observed against other fish pathogens and the closely related Vibrionaceae genera. This specific probe may be useful for rapid identification of V. anguillarum and V. ordalii.

引用

页码：167 / 171

页数：5

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