SPLIT INJECTOR FOR CAPILLARY ZONE ELECTROPHORESIS

A split injector is described for capillary zone electrophoresis. It consists of a pump for delivery of the medium, a rotary injector, an interface, and a fused-silica capillary connected between the interface and the rotary injector. Several factors affecting split injection are examined, such as geometrical configuration of the interface and sample loop volume. The use of this split injector is demonstrated for both rectangular capillaries with large cross-sectional areas and the more conventional circular capillaries. The present injection method requires no interruption of the applied voltage.