CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BACILLUS-STEAROTHERMOPHILUS FARNESYL DIPHOSPHATE SYNTHASE EXPRESSED IN ESCHERICHIA-COLI

被引：11
|
作者：
NAKANE, H
KOYAMA, T
OBATA, S
OSABE, M
TAKESHITA, A
NISHINO, T
OGURA, K
MIKI, K
机构：
[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN
[2] TOYOTA MOTOR CO LTD,BIORES LAB,TOYOTA,AICHI 471,JAPAN
[3] TOHOKU UNIV,INST CHEM REACT SCI,AOBA KU,SENDAI,MIYAGI 980,JAPAN
[4] TOHOKU UNIV,FAC ENGN,DEPT BIOCHEM & ENGN,AOBA KU,SENDAI,MIYAGI 980,JAPAN
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 04期
关键词：
FARNESYL DIPHOSPHATE SYNTHASE; THERMOSTABLE ENZYME; PRENYLTRANSFERASE; CRYSTALLIZATION; X-RAY DIFFRACTION;
D O I：
10.1006/jmbi.1993.1554
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 Å resolution. The diffraction pattern indicated that the space group is I4122 with unit-cell dimensions of a = b = 114 Å and c = 247 Å. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase. © 1993 Academic Press Limited.
引用
收藏
页码：787 / 788
页数：2
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