INTRASPECIFIC PROTOPLAST FUSION OF CANDIDA-TROPICALIS FOR ENHANCING PHENOL DEGRADATION

被引：3
|
作者：
CHANG, SY
LI, CT
HIANG, SY
CHANG, MC
机构：
[1] NATL CHENG KUNG UNIV,COLL MED,DEPT BIOCHEM,TAINAN 70101,TAIWAN
[2] CHIA NAN JR COLL PHARM,DEPT IND SAFETY & HYG,TAINAN,TAIWAN
[3] NATL CHENG KUNG UNIV,GRAD INST ENVIRONM ENGN,TAINAN 70101,TAIWAN
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1995年 / 43卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Auxotrophic mutants and phenol-degrading defective mutants were separately isolated in a phenolutilizing strain of Candida tropicalis M4, and were hybridized through protoplast fusion. Some protoplast fusants with phenol-degrading ability were obtained and were very stable. Two of the fusants exhibited slightly higher rates of growth than did the wild strain when the cells were grown on phenol medium, and they possessed about 1.9 and 2.2 times respectively higher phenol hydroxylase activity than the wild strain.
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页码：534 / 538
页数：5
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