CHROMOSOMAL MAPPING AND NUCLEOTIDE-SEQUENCE OF 2 TANDEM REPEATS OF ATLANTIC SALMON 5S RDNA

被引：284
|
作者：
PENDAS, AM
MORAN, P
FREIJE, JP
GARCIAVAZQUEZ, E
机构：
[1] Departamento de Biologia Funcional, Oviedo
来源：
CYTOGENETICS AND CELL GENETICS | 1994年 / 67卷 / 01期
关键词：
D O I：
10.1159/000133792
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Atlantic salmon 5S ribosomal DNA (5S rDNA) was amplified by the polymerase chain reaction, using as primers conserved sequences from the coding region of rainbow trout 5S rRNA. Two amplified products of different molecular weights were obtained, cloned, and sequenced, revealing them to be tandemly arranged. The nucleotide sequences differed between the two clones in the length of the nontranscribed spacer (NTS) and in three nucleotides of the coding sequence. By means of fluorescence in situ hybridization the 5S rDNA was chromosomally located in the heterochromatic arm of the pair bearing the satellite, adjacent to the major ribosomal DNA locus (rDNA).
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页码：31 / 36
页数：6
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